FastQCFastQC Report
Mon 27 Feb 2023
SRR3127419.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127419.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3190383
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402741.2623562750929904No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT135950.086.7988741
CGTAGGG10050.076.286392
TAGGGCA15600.072.904684
AGGGATG49050.072.818085
ATAGGGC18450.072.596073
AGTAGGG53900.070.422312
TAGACGG3550.068.946781
GGTAAGG21850.068.50381
ATAGGGA29600.067.795493
GAGGGAT42250.067.741564
GAATAGG17200.067.320241
TAGAGGG62650.066.96832
TAAGGGA25000.065.9829563
AAGAGGG121350.065.853542
AGATAGG16750.064.9136661
AGTAAGG21100.064.692581
TATAGGG24950.064.473472
TAGGGTC9400.063.9951024
GTAGGGA24700.063.740063
TAGGGAT24500.063.684924
Produced by FastQC (version 0.11.5)