FastQCFastQC Report
Mon 27 Feb 2023
SRR3127418.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127418.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3661272
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT598551.6348143486744497No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT199650.085.608411
TAGGGCA23200.072.735334
ATAGGGC21850.072.711683
ATAGGGA34050.071.231413
ACGGGTA5200.069.602744
AAGGGTA20900.068.819914
AGTAGGG60700.068.454582
CGTAGGG11500.068.258642
AATAGGG59150.067.467082
GAATAGG25750.067.014721
AGGGATG51200.066.9262545
AGGGCAT34600.066.838695
TAGAGGG69950.066.726832
TATAGGG33400.066.706912
TAAGGGA30400.066.022693
ATGAGGG61650.065.646122
GTAGGGT11900.065.569223
AGATAGG21500.064.734321
AAGAGGG133000.064.710632
ATAGCGG10200.064.5370561
Produced by FastQC (version 0.11.5)