FastQCFastQC Report
Mon 27 Feb 2023
SRR3127417.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127417.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3141355
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT488551.5552205974810234No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT160800.085.879281
TAGGGCA17200.072.909784
ATAGGGC16650.072.48673
AGTAGGG51150.070.417462
GAATAGG20200.070.301871
ATAGCGG7300.068.5069661
ATAGGGA26600.068.235713
ACGGGTA3050.068.0119864
CGTAGGG7950.067.603682
TAGGGTA9450.066.850644
ATAAGGG43700.066.671412
AGGGATG41750.066.296845
TATAGGG26250.066.092342
AAGGGTA17950.065.66114
AGGGCAT27500.065.6194465
GAGGGAT35600.065.419854
TAGAGGG57350.065.106512
TAAGGGA25150.065.046533
AGTAAGG20600.065.0433351
AGAGGGC45450.065.0378653
Produced by FastQC (version 0.11.5)