FastQCFastQC Report
Mon 27 Feb 2023
SRR3127416.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127416.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3199417
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT473571.480175919550343No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT159050.084.900771
TAGGGCA16750.074.630434
ATAGGGC18000.074.147233
ATAGGGA23700.071.582693
AGTAGGG53100.069.527552
ATAGCGG7700.068.4608151
GAATAGG21550.068.3615341
AGGGATG43850.067.625255
TAGAGGG57250.067.362812
AATAGGG44850.067.2167362
GTAGGGA23300.067.164133
TAGGGAT17850.066.872054
AGAGGGC47000.066.492543
AGGGCAT27650.066.4554755
CGTAGGG9300.066.2476042
AGTAAGG19550.065.965761
AGATAGG18700.065.943871
ATAAGGG44150.065.619342
TAGCGGG16400.065.38422
AAGAGGG110350.065.250622
Produced by FastQC (version 0.11.5)