FastQCFastQC Report
Mon 27 Feb 2023
SRR3127415.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127415.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2110854
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268201.2705757953889754No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC54420.2578103459547652No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26350.12483099257456934No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83300.085.51871
ATAGGGC10900.073.307983
TAGGGCA11700.072.714594
ATAGGGA20200.071.435783
AGGGTAT13900.071.350415
GAATAGG13650.071.306841
AGGGATG29450.070.066115
AGTAGGG33800.069.948782
TACGACC9850.069.669946
AGGGAAT25950.069.372965
CTACGAC9900.069.318085
TAGCGGG10650.069.291322
CGTAGGG5950.068.727592
AGTAAGG13050.068.4599461
AAGGGTA16350.068.13334
AGATAGG10350.067.692321
TAAGGGA16200.066.7331543
TCCGCTA10500.066.725291
TATAGGG18250.066.448582
AGGGCAT16950.066.2761155
Produced by FastQC (version 0.11.5)