FastQCFastQC Report
Mon 27 Feb 2023
SRR3127414.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127414.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816614
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223201.2286594730636229No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45510.250521024279236No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG27090.1491235892710284No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22190.12215033022975712No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG21940.12077414354397797No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC19190.10563609000040736Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66850.086.293521
ATAGGGC9800.075.526413
TATAGGG14600.072.330332
TACGACC8500.071.319326
AGGGAAT20700.071.284615
ATAGGGA15750.071.239633
TAGGGCA8650.070.8520664
TAGCGGG9000.070.715812
CTACGAC8600.070.4900365
TAGGGTA6850.070.199644
ACGACCA8700.069.67987
AGGGTAT9800.069.530985
AGATAGG8550.068.959971
AAGGGTA13200.068.930064
GGTAAGG9050.068.798431
AGTAGGG28400.068.3918152
GTAGGGT7800.068.298173
AAGAGGG61400.068.1820142
AGGGTAA10250.067.395355
AGGGATG26050.067.287995
Produced by FastQC (version 0.11.5)