FastQCFastQC Report
Mon 27 Feb 2023
SRR3127413.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127413.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1863617
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215421.1559242054563788No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC47080.2526270151002057No Hit
GCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG28940.15528941837298116No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23430.125723257514822No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG22500.12073296176199294No Hit
CTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTGC20060.10764014279758126Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66100.083.595741
TAGGGCA10000.078.953944
ATAGGGC11800.076.0704353
TACGGGT1200.074.410953
TAGCGGG9550.072.4002152
AGGGATG26950.072.194835
AGTAGGG29250.071.879912
ATAGGGA17600.070.494583
TATAGGG13250.070.287032
TACGACC9550.068.895236
AGGGAAT23700.068.610765
AGGGTAC9800.068.576375
AGATAGG10900.068.225721
TAGGGTA5900.067.706674
GGTAAGG10150.067.238721
AGGGTAT10150.066.674685
GAATAGG12200.066.356911
CTACGAC9950.066.125575
TAGAGGG32450.066.096132
AGACGGG13200.065.920982
Produced by FastQC (version 0.11.5)