FastQCFastQC Report
Mon 27 Feb 2023
SRR3127412.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127412.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2759435
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331181.20017322386648No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC59810.216747268915557No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32160.11654559719652756No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102900.086.227521
TAGGGCA15150.074.155064
AGTAGGG48950.071.445652
TAGAGGG53050.070.354312
GTAGGGA21700.069.317963
ATAGGGC20050.068.457973
AGGGATG41050.068.2477955
TAAGGGA22400.067.3616263
TATAGGG25450.066.3074342
AGGGTAT14100.066.0087665
TAGGGTC7350.065.8726964
ATAGGGA24700.065.466193
CGTAGGG8050.065.399992
GGTAAGG16250.065.394991
TAGGGAT18500.065.046484
AGGGCAT25300.064.656815
ATAAGGG45900.064.620792
AAGAGGG98650.064.517442
GAGGGAT34050.064.33164
GTAGGGT11600.064.025743
Produced by FastQC (version 0.11.5)