FastQCFastQC Report
Mon 27 Feb 2023
SRR3127411.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127411.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2403641
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279461.1626528254427346No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC51510.21429988920974472No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26840.11166392984642882No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG26210.10904290615778313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83800.087.236831
TAGGGCA13350.074.855224
ATAGGGA22500.073.7441563
ATAGGGC15600.073.728043
TAGAGGG44150.071.320312
AGGGATG36000.070.620735
AGTAGGG43550.069.921682
AGATAGG15400.069.521221
GTAGGGA18750.069.386543
TAGGGAT15250.068.3108754
ATAGAGG18000.067.8641
CGTAGGG6600.067.849582
TATAGGG19100.067.621482
GAGGGAT31800.067.297084
AGGGAAT22100.067.194275
TAAACGG2550.066.584721
GAATAGG12850.066.433581
GGTAAGG14900.066.1564561
AGGGATA24500.065.407225
GTAGGGT10250.065.302823
Produced by FastQC (version 0.11.5)