FastQCFastQC Report
Mon 27 Feb 2023
SRR3127410.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127410.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2483149
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270141.087892832850546No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC51710.2082436454679119No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28680.11549850613072352No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG27070.10901480338070732No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81100.086.39321
TAGGGCA13000.074.473984
AGGGATG38850.074.035695
CTACGAC11200.072.5953455
GTAGGGA19150.072.153813
AGTAGGG43500.072.098672
TAGGGAT15750.069.229024
TAAGGGA19700.068.946523
GGTAAGG15000.068.70031
ATAGGGA20550.068.381733
TACGACC11900.068.325046
TAGAGGG45900.068.1239242
AGATAGG15900.067.475091
CGTAGGG7700.067.1726762
TATAGGG19350.066.825532
GAGGGAT31700.066.42014
ATAGAGG16600.066.3305361
AGGGAAT23200.066.243085
GATAGGG39000.066.190922
AGGGACT30300.066.076615
Produced by FastQC (version 0.11.5)