FastQCFastQC Report
Mon 27 Feb 2023
SRR3127409.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127409.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581854
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119452.0529204920822064No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC42640.7328298851601949No Hit
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG15050.2586559514929862TruSeq Adapter, Index 16 (100% over 22bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC14740.25332815448548945TruSeq Adapter, Index 16 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG14590.25075018819153944TruSeq Adapter, Index 16 (100% over 22bp)
TAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATA6200.10655594014993452No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG450.094.03871
ATAGCGG1100.089.7642061
CGTTTTT38750.087.8503341
ACGGGTA500.084.598454
AGGGATG15400.084.537425
ATAACGG452.7284841E-1183.589951
CAGGGTA1350.083.554034
TAAGGGA5700.082.4546363
AGGGAAT9700.081.88515
AGGGTAT4050.081.2330865
AGGGTAC2200.081.1803365
GGAATGC8450.080.656347
CGCTACG7100.080.097133
AAGGGCA9400.079.498554
TAAGGGC5450.079.3380053
AAGGGCG2800.078.891414
CGACCAA7150.078.886458
AGTAAGG3050.078.622511
GGATGAC7000.078.562467
GCTACGA7250.078.439944
Produced by FastQC (version 0.11.5)