FastQCFastQC Report
Mon 27 Feb 2023
SRR3127408.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127408.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551484
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97861.7744848445285812No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37800.6854233305046021No Hit
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG24520.44461852021092185TruSeq Adapter, Index 16 (100% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG24120.43736536327436515TruSeq Adapter, Index 16 (100% over 22bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC23260.4217710758607684TruSeq Adapter, Index 16 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT7710.13980459995212915TruSeq Adapter, Index 16 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT7570.13726599502433434TruSeq Adapter, Index 16 (95% over 22bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTACCCTC7060.12801821993022464No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTT6850.12421031253853239No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCTA6720.12185303653415148No Hit
AGAGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTT6390.11586918206149227No Hit
ACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG6120.11097330112931653TruSeq Adapter, Index 16 (95% over 23bp)
GACACACCCACACACCTGTCTCTTATACACATCTGACGCCTACCCTCTCG6060.10988532758883303No Hit
GGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT5940.10770938050786605TruSeq Adapter, Index 16 (100% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTT5840.10589609127372689No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG1450.094.302672
CGTTTTT33750.088.598851
AGGGTAC1600.088.112165
GCGATTA650.086.756598
AGTAAGG2300.086.1259461
AGGGATG15150.085.30115
CGCTACG6250.083.740773
GGATGAC6900.082.408297
CGAGGGA2600.081.608083
TAAGGGA5650.080.950083
AATACGG353.7685822E-880.8529361
CAGGGAT4850.080.691984
GCATAGG1000.080.179161
CGGGATG2350.079.988355
GGGATGA8600.079.779086
GGGCGAT2300.079.684056
CTACGAC6550.079.637255
CAGGGCA3150.079.3344
GCTACGA6600.079.299974
TAGACGG1250.079.235881
Produced by FastQC (version 0.11.5)