FastQCFastQC Report
Mon 27 Feb 2023
SRR3127407.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127407.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565885
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95491.6874453289979412No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC37970.6709843872871697No Hit
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG25260.4463804483243062TruSeq Adapter, Index 16 (100% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG24690.43630773036924464TruSeq Adapter, Index 16 (100% over 22bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC23120.40856357740530325TruSeq Adapter, Index 16 (95% over 24bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTACCCTC18440.3258612615637453No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA9550.168762204334803No Hit
AGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT8960.15833605767956385TruSeq Adapter, Index 16 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT8600.1519743410763671TruSeq Adapter, Index 16 (95% over 22bp)
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCTA7210.12741104641402407No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTT7100.12546718856304725No Hit
GAATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTT6910.12210961591136009No Hit
ACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG6720.11875204325967291TruSeq Adapter, Index 16 (95% over 23bp)
GACACACCCACACACCTGTCTCTTATACACATCTGACGCCTACCCTCTCG6330.11186018360620975No Hit
AGAGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTT6100.10779575355416736No Hit
GGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT5960.10532175265292418TruSeq Adapter, Index 16 (100% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT5690.10055046520052661TruSeq Adapter, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG156.902889E-493.991438
GACCGAT500.093.9914258
CGTTTTT30400.089.4248051
AGGGATG15800.086.8528445
GGATGAC6800.083.6247257
ATAGCGG1350.083.6144261
GGGATGA8050.082.899276
GGTAAGG2100.082.8678741
ACCGATG401.035005E-982.24259
GATCTCG401.035005E-982.24258
AGGGCAT6250.081.9605265
CGCTACG6200.081.86353
GCTACGA6150.081.76494
GAGGGAT10900.081.4879764
CGACCAA6000.080.675978
ATCTAGG353.8422513E-880.6282041
TGCACGG353.8422513E-880.6282041
CGGGTAT700.080.564085
CTACGAC6300.079.8181155
GATGGCA13250.079.4493568
Produced by FastQC (version 0.11.5)