FastQCFastQC Report
Mon 27 Feb 2023
SRR3127405.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127405.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908440
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235771.2354069292196768No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC22370.11721615560352959No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71750.085.038311
CGTAGGG5850.074.914672
ATAGGGC13150.073.821223
TAGGGCA10000.073.0417944
AGGGCAT16650.070.843965
GTAGGGA12250.070.012423
AGTAGGG29350.070.003242
AGATAGG12350.068.72391
TAGAGGG33950.068.568862
CGAAGGG20700.068.522932
TAGGGCG3750.067.858194
ATAGAGG13000.066.738551
AAGGGTA13000.066.698234
TAGGGAT9900.066.639644
AAGGGCA30450.066.5458454
ATGAGGG33000.066.4016342
GAATAGG12150.066.3624041
AGAGGGC24650.066.3364643
ATAGGGT8250.066.2588353
GATAGGG31700.065.7056662
Produced by FastQC (version 0.11.5)