FastQCFastQC Report
Mon 27 Feb 2023
SRR3127404.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127404.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1936660
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227391.1741348507223777No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC23640.12206582466721055No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG19810.10228950874185452No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG19560.10099862650129604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75400.084.6258241
CGTAGGG6550.075.372812
ATAGGGC10100.074.449783
ACGGGAT4550.070.236414
AGTAGGG29000.070.0410162
TAGGGTA7450.068.129044
AGGGTAC8150.068.04395
TAGGGCA10000.067.674854
AGTAAGG11900.067.222791
AGATAGG11800.066.1973651
TAGAGGG33450.066.0645452
AGGGCAT17650.065.7683645
AGAAGGG64500.065.6068952
GAGGGAT24950.065.5501254
GTAGGGT8400.065.4593053
TAGGGTC6250.065.419024
TAGGGCG3600.065.272814
CGAAGGG23950.065.1777342
ATAGAGG13450.065.073661
ATAAGGG27000.064.60662
Produced by FastQC (version 0.11.5)