FastQCFastQC Report
Mon 27 Feb 2023
SRR3127403.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127403.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527114
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90651.7197418395261745No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23830.45208436884620784No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG14660.27811820592888825No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC11850.2248090545878121No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG11280.21399545449371482No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA8280.1570817697879396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG301.3184035E-894.063321
GCGATTG156.8972714E-494.009788
TACGGGT254.9144364E-794.009773
GCGGATA254.9144364E-794.009774
CGTAGGG850.088.479792
CGTTTTT37350.085.75251
TAAGGGA4700.085.008843
ATAGCGG950.084.161911
GATGCGG850.082.997051
GATTAGG850.082.997051
AGGGATG11900.082.55485
GGATGGC11700.081.957237
ATTGCGG353.842615E-880.62571
GGGTATC2250.079.386036
ATAACGG551.8189894E-1276.960891
GGGATGG13700.075.82546
GATGGCA11750.075.607868
ATAAGGG7650.075.5764852
CTACGAC3950.074.969825
GCTACGA3950.074.969824
Produced by FastQC (version 0.11.5)