FastQCFastQC Report
Mon 27 Feb 2023
SRR3127402.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127402.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481977
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75261.5614852990910355No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21390.44379711065050825No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG19120.3966994275660457No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC17230.3574859381256782No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG16100.3340408359735008No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCCCATGG7950.16494563018567276No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA6680.13859582511198668No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCTCCCATGGTCG6620.13735095243134007No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT6410.13299389804907702No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG5620.11660307442056363No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG5480.11369837149905493No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC4970.10311695371355895No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT4900.1016646022528046No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGG500.094.320781
ACGGGTC301.2998498E-894.252084
AATCGGG201.8125435E-594.2520752
CGTTTTT34550.086.949831
CGGGTAT600.086.155725
GGATGGC10350.085.815197
AACGAGG1050.085.337841
AGGGATG12150.084.705295
AGAGCGG950.084.3922651
TACGACC3800.084.094596
TAAGGGA3150.083.7796253
CAACCGG401.0059011E-982.530681
CGCTACG3900.082.168483
CGAGGGA2250.081.6851353
TCCGCTA3950.081.18751
TAGGGCA1800.081.1615144
GGGATGG12050.080.728356
ATAGCGG1000.080.172661
CTACGAC4050.080.063915
AAGGGAC3450.079.226394
Produced by FastQC (version 0.11.5)