FastQCFastQC Report
Mon 27 Feb 2023
SRR3127401.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127401.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497449
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71491.4371322487330358No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22040.44306049464367203No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCCCATGG20390.4098912652352301No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG19600.3940102402457337No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC18660.3751138307645608No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG16880.33933126812999925No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA10010.201226658411214No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA7030.1413210198432402No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCTCCCATGGTCG6780.13629537902377933No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT6220.12503794358818693No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG5960.1198112771359476No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG5610.11277537998870237No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATG5520.11096614929369643No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT5440.10935794423146895No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC5420.10895589296591207No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGG156.864466E-494.12181
ATTGCGG450.094.12181
AACGGGT254.9203845E-793.989353
GCGGATA156.9030025E-493.989354
CGTTTTT30050.088.014071
CAGGGTA1150.085.816364
TAAGGGT1150.085.816363
CGCTACG4000.084.5904163
AGGGATG12750.084.406115
GGATGGC11500.084.181767
TAACGGG1300.083.1945952
CGGGAAT1700.082.931785
AAGGGAT9600.082.730214
ATAGCGG750.081.5722351
GCTACGA4150.081.532934
TCCGCTA4050.081.339831
TAGCGGG2400.080.331012
CGTAGGG1000.079.939162
TAAGGGA3700.078.7478263
CTACGAC4300.078.688755
Produced by FastQC (version 0.11.5)