FastQCFastQC Report
Mon 27 Feb 2023
SRR3127400.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127400.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2270315
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326841.4396240169315713No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG30910.13614850802641923TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC28900.12729511103084815TruSeq Adapter, Index 20 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28210.12425588519654762No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG28080.12368327743066491TruSeq Adapter, Index 22 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26680.11751673225962037No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90100.086.792941
TAGGGCA13850.076.6997154
CGTAGGG7050.076.006522
ATAGGGC14950.071.685083
AGTAGGG38350.070.107712
GAATAGG13200.069.823091
GTAGGGT10400.069.602133
TAGAGGG38750.068.1710052
AAGGGTA15600.067.191664
ATAGGGA17750.066.99733
TGTAGGG16300.066.6130752
ACGGGAT6950.066.279074
AGGGCAT18800.066.255685
GTAGGGC12300.066.111373
AGGGATG29000.065.967735
AAGAGGG77900.065.829782
TAGGGTA9950.065.6639254
GTAGGGA13350.065.136673
ATGCGGG12300.064.964922
ATGAGGG39650.064.726862
Produced by FastQC (version 0.11.5)