FastQCFastQC Report
Mon 27 Feb 2023
SRR3127399.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127399.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1984479
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256131.2906662151627706No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG41470.20897172507242456TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC33510.16886044145591864TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG32870.16563541362745587TruSeq Adapter, Index 22 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24400.1229541859601437No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23120.11650413030321813No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75250.086.544921
CGTAGGG5000.078.226722
TAGGGCA12200.073.390674
ATAGGGC12550.072.8458943
AGTAGGG32000.072.306712
ATGCGGG9900.071.400812
AGTAAGG12100.071.321451
GTAGGGA13150.071.3139343
AGTACGG2450.071.218051
GTAGGGT7850.070.836883
CGAAGGG17250.068.8428042
TAGAGGG34050.068.5070342
AGGGTAC8600.068.305135
TACGACC5000.067.671266
GAATAGG11250.067.488191
AAGGGTA14250.067.4624944
GGTAAGG11350.066.4780961
AAGAGGG62600.066.471192
TAGACGG2200.066.449761
ATAGCGG3550.066.419571
Produced by FastQC (version 0.11.5)