FastQCFastQC Report
Mon 27 Feb 2023
SRR3127395.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127395.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2622066
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280171.068508573010748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89850.084.917591
TAGGGCA11850.074.161134
ATAGGGC14550.072.672933
TAGAGGG44450.071.099322
AGTAGGG40900.070.3713762
ATAGGGA20750.069.983153
GTACGGG5650.069.919572
ATAGCGG6150.069.6464541
AGGGCAT23300.067.366455
TAGGGAT15200.066.782594
GAATAGG14700.066.6006851
TAGTAGG11700.066.379011
AGGGATG32250.066.0117955
TATAGGG19500.065.5997542
CGTAGGG8200.065.38212
AAGAGGG101100.064.845452
AATAGGG38100.064.804072
TAGCGGG15750.064.795842
GGTAAGG16000.064.131181
TAAGGGA22100.064.006973
Produced by FastQC (version 0.11.5)