Basic Statistics
Measure | Value |
---|---|
Filename | SRR3127394.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1625157 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 25837 | 1.5898156301206592 | No Hit |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 6387 | 0.393008183209376 | No Hit |
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC | 3041 | 0.18712038283070498 | TruSeq Adapter, Index 23 (96% over 25bp) |
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG | 2998 | 0.1844744846190245 | Illumina PCR Primer Index 7 (95% over 22bp) |
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG | 2998 | 0.1844744846190245 | Illumina PCR Primer Index 7 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 7700 | 0.0 | 86.33738 | 1 |
CGTAGGG | 535 | 0.0 | 81.71765 | 2 |
CTACGAC | 1095 | 0.0 | 79.85195 | 5 |
TCCGCTA | 1160 | 0.0 | 77.007996 | 1 |
AGGGATG | 2835 | 0.0 | 76.774086 | 5 |
ATAGCGG | 260 | 0.0 | 75.94796 | 1 |
AGGGTAC | 750 | 0.0 | 73.9618 | 5 |
TACGACC | 1195 | 0.0 | 73.56317 | 6 |
CGCTACG | 1195 | 0.0 | 73.169785 | 3 |
GTACGGG | 310 | 0.0 | 72.789085 | 2 |
TAGGGCA | 990 | 0.0 | 72.65123 | 4 |
GCTACGA | 1210 | 0.0 | 72.26272 | 4 |
ACGACCA | 1210 | 0.0 | 72.26272 | 7 |
AGGGCAT | 1305 | 0.0 | 72.045395 | 5 |
AGTAGGG | 2455 | 0.0 | 71.806946 | 2 |
GAGGGTA | 1055 | 0.0 | 70.40303 | 4 |
GTAGGGC | 675 | 0.0 | 70.34031 | 3 |
CGGGATG | 635 | 0.0 | 70.32935 | 5 |
CGAGGGA | 955 | 0.0 | 69.89912 | 3 |
TAGCGGG | 1045 | 0.0 | 69.72719 | 2 |