FastQCFastQC Report
Mon 27 Feb 2023
SRR3127392.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127392.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1471188
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200251.3611448706759435No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC57010.38750995793875426No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG42970.29207687936551957Illumina PCR Primer Index 7 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG38280.2601978808962553Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC37300.253536597634021TruSeq Adapter, Index 23 (96% over 25bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAAGTCTTC21960.14926712289659785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60400.086.407021
CGTAGGG4600.081.791062
TAGGGCA7500.081.460244
CTACGAC11450.081.26875
TCCGCTA11900.079.09291
CGCTACG11850.078.922053
AGGGCAT11150.077.975925
GCTACGA12150.076.973364
TACGACC12200.075.887466
AGGGATG24650.075.117815
ACGGGTA1450.074.545854
CGGGATG4800.074.4108055
AGAGGGC17100.074.204683
ACGACCA12300.073.742167
GTAGGGA10750.073.445373
AGTAGGG19900.073.0262762
AGGGTAC6050.073.0190355
GGTAAGG9100.072.400421
TAGCGGG6800.071.236412
CGACCAA12750.071.139498
Produced by FastQC (version 0.11.5)