FastQCFastQC Report
Mon 27 Feb 2023
SRR3127390.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127390.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1737563
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209361.2049059516115388No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC60580.3486492288337171No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG31450.1810006313440146No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG25420.1462968536968156No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC24420.1405416666906466No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC18850.10848527506628536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65850.087.299971
ATAGCGG2950.079.93081
AGGGATG41550.079.623355
ACGGGTA2500.079.188534
GGATGGC32550.076.08497
AGTAAGG11600.074.397651
CTACGAC9850.072.9951555
TAAGGGA18350.072.4379353
CGAGGGA10350.072.411873
AGGGTAT11100.072.395565
ACGACCA9900.071.6771247
AGGGAAT25900.071.488355
TAGGGCA10700.071.364844
AAGGGAT38250.071.351014
AGGGCAT16850.071.117935
GGGTATC6300.070.863386
ATAGGGA20000.070.704053
TACGGGT1800.070.704053
CGACCAA10250.070.146568
GGGCGAT5100.070.02976