FastQCFastQC Report
Mon 27 Feb 2023
SRR3127389.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127389.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1771994
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199191.1241008716733805No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC61770.34859034511403536No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG32860.18544080849032218No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG26670.15050841029935766No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC24100.13600497518614624No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC18940.10688523776039872No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65900.085.939421
ATAGCGG3850.081.926861
CTACGAC11800.078.8538065
AGGGATG37600.078.489455
TCCGCTA12200.077.176011
GGATGGC33000.075.474697
ACGGGTA2450.074.806264
CGAGGGA10650.074.5721363
CGACCAA12400.074.280338
ACGACCA12400.073.901357
CGCTACG12650.073.555323
CGTAGGG4750.073.277372
AGGGCAT18000.072.3180545
AGTAAGG12400.071.375371
GAGGGAT32000.071.371654
CTAGCGG1650.071.3293461
GCGAGGG15300.071.322792
TAGGGTA7150.070.983464
ATGCGGG9350.070.931522
TAAGGGA17500.070.893323
Produced by FastQC (version 0.11.5)