FastQCFastQC Report
Mon 27 Feb 2023
SRR3127388.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127388.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3539047
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520151.469745951381827No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39000.11019915813494424No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT159950.085.5913541
CGTAGGG10400.072.76822
ATAGGGC21500.072.585323
GTACGGG8550.070.920652
ATAGCGG8850.070.653081
AGGGATG56450.068.947025
TAGGGCA21200.068.069354
AGAGGGC53650.067.551313
GAATAGG21650.067.3172761
AAGAGGG128150.067.197942
AGGGCAT31350.066.722395
ATGCGGG18100.066.482982
ATAGGGA35600.066.4151463
GGTAAGG22550.065.672991
TGTAGGG28350.065.658542
TAGAGGG68500.065.3274152
TAAGGGA33350.064.976183
GTAGGGT13200.064.810683
CGAAGGG43000.064.714622
ACGGGTA4800.064.632634
Produced by FastQC (version 0.11.5)