FastQCFastQC Report
Mon 27 Feb 2023
SRR3127387.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127387.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3035731
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421221.387540595658838No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32300.10639941417734312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT124850.085.567981
ATAGCGG7600.075.060551
TAGGGCA15900.071.716894
GAATAGG19100.071.582121
ATAGGGC18800.071.4315643
CGTAGGG7450.070.8377762
TAGAGGG58100.069.665892
AGGGATG47700.069.161055
ATAGGGA28800.069.0435643
TAGCGGG18800.067.672012
ATAGAGG22700.067.4990161
TAGGGTA10550.067.4415744
AGAGGGC48350.067.244353
AGGGCAT26650.067.184735
AAGAGGG111150.066.5993352
ACTAGGG17000.065.967682
ACGGGTA4150.065.854144
AGTAAGG19700.065.572881
AATGCGG7700.065.513831
AAGGGGC90050.065.250824
Produced by FastQC (version 0.11.5)