FastQCFastQC Report
Mon 27 Feb 2023
SRR3127385.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127385.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2975844
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT356641.1984499187457407No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC56530.18996291472268034No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30220.10155102216379622No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT106550.086.05721
AGGGATG47800.072.869045
ATAGGGC15250.071.202443
TAGAGGG53650.070.530822
AGTAGGG47500.070.162552
TAGGGCA16950.070.162264
ATAGGGA27700.068.896793
CGTAGGG7450.068.7737962
GTAGGGA22550.067.9553763
GTAGGGT11900.067.941363
GAGGGAT40350.067.800394
GTACGGG6450.066.318462
TAGGGTA11000.066.235644
TATAGGG24000.066.2000352
TGTAGGG25000.065.996342
TAAGGGA28650.065.9559863
ATAGAGG23600.065.7518541
GATAGGG46900.064.946372
TAGGGAT24450.064.597124
AAGAGGG104200.064.238452
Produced by FastQC (version 0.11.5)