FastQCFastQC Report
Mon 27 Feb 2023
SRR3127384.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127384.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2605837
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295471.1338775218864419No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC49600.1903419131741548No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG33030.12675389903512768No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27300.10476480301722634No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT88650.086.814281
GTACGGG5650.075.083272
CGTAGGG6000.073.845782
AGGGATG39650.073.127515
AGTACGG3400.072.123061
GTAGGGA21050.071.8793
GAGGGAT32850.069.160924
AGTAGGG42900.068.670762
TAGGGCA12850.068.227414
TAGAGGG43600.067.892572
AAGGGCG11950.067.843724
TAAGCGG6150.067.477241
TAGGGAT19650.067.405124
TAGGGTA10050.067.068574
TGTAGGG20950.066.822322
AAGGGTA20100.066.599564
ATAGGGA24500.066.567023
AAGAGGG87900.066.547542
AGGGATA27450.066.4244845
AAAGGGT24900.065.686973
Produced by FastQC (version 0.11.5)