FastQCFastQC Report
Mon 27 Feb 2023
SRR3127383.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127383.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2657472
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286951.0797856007513909No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC50680.19070755966572742No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG34960.1315535968017725No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27640.10400862172771717No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC27370.1029926185487561TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83600.086.122211
AGGGATG41250.074.508065
GTAGGGA19700.074.18973
CGTAGGG6300.070.910482
ACGGGTA2850.070.904374
TAGGGTA10600.070.048794
GAGGGAT34850.068.772824
AGTAGGG41100.068.420582
TAGAGGG49450.068.278742
GTACGGG5450.068.164452
TGTAGGG21450.067.961312
AAGAGGG87700.067.1323852
TAGGGCA12800.066.820674
ATGAGGG44600.066.741542
AAGGGTC20000.065.792664
ATAGGGA22800.065.7514343
ATAGGGC14250.065.627773
TAAGGGA23500.065.192733
ATAGAGG20650.064.946361
ATGTAGG9150.064.800661
Produced by FastQC (version 0.11.5)