FastQCFastQC Report
Mon 27 Feb 2023
SRR3127382.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127382.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1855305
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234021.261355949560854No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23280.12547802113399142No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21730.11712359962378154No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75450.085.3980941
CGTAGGG5050.078.192292
ATAACGG1700.074.696751
AAGGGTA14150.073.751794
AGTAGGG31250.070.851162
ATAGGGC11100.070.300953
AGGGTAC10400.069.1566855
AGGGCAT18150.068.3759465
TAGGGCA9700.068.3318944
TAGCGGG11450.068.152012
AGATAGG10950.067.432991
GTAGGGA12600.067.154953
GATAGGG27500.066.4956052
ATAGGGA16700.066.1496353
AATAGGG28350.065.994252
ATAGCGG5850.065.9241941
AGGGTAT13000.065.811855
AGGGATG24200.065.46225
TAAGGGA14400.065.2895363
GAATAGG11600.064.8707351
Produced by FastQC (version 0.11.5)