FastQCFastQC Report
Mon 27 Feb 2023
SRR3127380.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127380.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638355
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188781.1522533272703412No Hit
GCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTG22150.1351965843788434No Hit
CCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTG21190.12933704844188226No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT21030.12836045911905541No Hit
CTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC20020.12219573901871084No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC19480.11889975005417018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57350.085.7623751
ATGTAGG4900.073.001441
CGTAGGG4050.071.997742
TAGGGTA7200.071.149414
TAGGGCA8050.070.642624
ACGGGTA1800.070.496664
AGGGTAC8950.069.3151555
AGGGCAT16750.069.304185
GTAGGGA10550.068.603383
GTAGGGT7000.068.482473
ATAGGGC10150.068.0657353
AGGGTAT11400.068.023095
TATAGGG12800.067.974182
TAGGGAC6650.067.8464054
CGAAGGG15700.067.700362
AAGGGTA12250.067.523344
TAGCGGG10100.067.519442
TAGAGGG30950.067.469052
AGTAGGG27100.066.9884952
ATAGAGG12150.066.629431
Produced by FastQC (version 0.11.5)