FastQCFastQC Report
Mon 27 Feb 2023
SRR3127379.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127379.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3605586
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT422051.17054481573869No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC72030.1997733516826391No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT140900.084.9242551
ATAGCGG6900.081.76871
AGTAGGG54700.072.274532
TAGGGCA16350.071.5909654
CGTAGGG10050.070.629722
AGGGTAT20900.070.6252445
AGGGCAT36350.070.480465
TAGAGGG69900.069.87392
GAATAGG23850.069.786251
TACGGGT3100.069.7545853
ATAGGGA37800.069.51793
AAGGGTA26600.069.275724
AGGGTAC19000.069.0283055
TAGGGTA12400.068.617284
CGAAGGG38150.068.264022
TAAGGGA31400.068.117453
AGTAAGG21500.067.354581
AGAGGGC54550.067.216583
CTACGAC14650.066.42165
TATAGGG29300.066.42162
Produced by FastQC (version 0.11.5)