FastQCFastQC Report
Mon 27 Feb 2023
SRR3127377.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127377.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3176337
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323861.0196021391936687No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC63920.20123809280942168No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC39510.12438856456352082TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG37500.11806052065634093No Hit
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG36270.11418813557881297No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT108500.084.887461
ATAGCGG6000.077.654461
TAGGGCA14100.077.324144
TACGGGT2550.075.559653
CGTAGGG8550.073.1369552
ACGGGAT7500.072.684694
TAGAGGG55350.071.69292
ATAGGGC21950.070.866293
AGGGTAC15850.070.269255
AGGGCAT29550.070.13385
GAATAGG21150.070.094291
AGTAGGG48750.069.729262
AGGGTAT17100.068.98015
AAGGGTA23200.068.668564
AAGGGCA48600.067.880824
AGTAAGG20000.067.771171
AAGAGGG113050.067.457722
CTACGAC13050.067.340655
CGAAGGG30400.067.277062
TAGGGTA10650.066.630584
Produced by FastQC (version 0.11.5)