FastQCFastQC Report
Mon 27 Feb 2023
SRR3127376.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127376.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2742226
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334241.2188637989720759No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30630.11169757707789219No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30390.11082237569040626No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT103250.085.934251
GTACGGG7050.075.343712
ACGGGTA1950.074.728244
GTAGGGA19450.074.436993
ATAGGGA27450.073.634923
TAGAGGG52950.072.440572
CGTAGGG7750.070.358082
AGGGATG38100.069.707765
AGTAGGG46200.069.4922562
ACGGGAT8650.068.471864
GAGGGAT34350.067.19124
AGTACGG4900.067.171711
TAGGGAC13600.067.053354
TAGGGCA17850.066.625434
TAGGGAT17600.066.503484
TAGGGTA8950.066.176714
AGGGCAT24950.066.129345
TAAGGGA26700.066.0203253
AAGAGGG100500.065.8558352
ATAAGGG40700.064.90822
Produced by FastQC (version 0.11.5)