FastQCFastQC Report
Mon 27 Feb 2023
SRR3127375.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127375.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2369140
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264871.118000624699258No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG30730.12970951484504925No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27580.11641355090876858No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26060.10999772069189663No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC25950.10953341718935984No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG24240.10231560819537891No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77750.085.319891
GTACGGG5750.078.675472
ACGGGTA950.074.405264
ATAGCGG4300.072.3655851
AGTACGG3950.071.616111
CGATCGG200.002136586570.720921
GTAGGGT9500.070.436973
TAGAGGG44700.070.21062
ATAGGGA20950.070.1788943
TAAGGGA22100.070.1519243
GTAGGGA17850.069.695013
AGTAGGG39700.069.319962
TAGGGTA7650.068.375024
CGTAGGG6000.068.328832
AGGGATG33500.067.054185
TAGGGCA14400.066.758054
AGAGGGC33050.066.585763
AGGGAAT22200.066.469055
GGTAAGG16000.066.300861
ATAACGG2800.065.669421
Produced by FastQC (version 0.11.5)