FastQCFastQC Report
Mon 27 Feb 2023
SRR3127374.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127374.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2415728
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260141.0768596464502627No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG31140.12890524098739595No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC28070.11619685659974965No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27400.11342336554446526No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27080.11209871310015035No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG25710.10642754482292709No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77900.085.929011
GTACGGG5750.076.897762
ACGGGTA2200.072.63124
AGTACGG3500.071.282931
AGTAGGG40000.071.263362
TAGAGGG43550.070.3147662
TAGGGCA13800.070.1544344
CGGGTAT2150.069.948515
ATAGGGA22950.069.41993
GTAGGGA18350.069.40653
ATAGAGG15400.069.387751
AGGGATG35700.069.244385
ATAGCGG5450.069.098911
TAGGGTA8950.067.7382054
ATAACGG2800.067.248041
GAGGGAT28600.067.044184
AGGGAAT26150.066.496225
CGAAGGG23800.066.4073262
AGTAAGG15000.066.21691
CGTAGGG5700.066.0189742
Produced by FastQC (version 0.11.5)