FastQCFastQC Report
Mon 27 Feb 2023
SRR3127372.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127372.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2834125
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421911.488678163454329No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT132500.087.5560461
AGGGATG44100.073.632745
CGTAGGG8000.071.905082
ATAGGGA25600.071.094673
ATAGGGC17150.070.657583
ATAGAGG19500.070.162791
TAGAGGG53150.069.8172
TAGGGCA15100.068.384384
GAGGGAT33500.067.277964
TAGGGAT19000.066.259384
AAGGGGC82000.066.011224
TATAGGG23400.065.890332
GATAGGG42850.065.472042
AATAGGG43100.065.201662
AAGAGGG100950.064.2223362
AGTAGGG43700.064.1985552
AGAGGGA66150.064.0082863
AGTAAGG18800.063.992021
AGAGGGC43750.063.9096373
AGGGTAT16550.063.887525
Produced by FastQC (version 0.11.5)