FastQCFastQC Report
Mon 27 Feb 2023
SRR3127370.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127370.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3017537
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327331.0847588612832253No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC46950.15559046997601023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT113600.085.507971
AGGGATG53400.071.563035
TAGGGTA10350.069.939064
TACGGGT3700.069.871553
TAGAGGG56500.069.466832
CGTAGGG7850.068.860092
ACGGGTA4100.068.787074
TATAGGG26100.068.255582
TAGGGCA18350.068.1373144
AGTAGGG55800.067.4742052
TAAGGGA28050.067.197163
AGGGCAT30600.067.127345
GAGGGAT49150.066.753084
ATAGGGA29250.066.690153
GAATAGG20250.066.172031
AGAAGGG117100.065.4691242
ATAGCGG6400.065.382971
AAGAGGG117500.065.086232
AAGGGAT62500.065.054234
GTAGGGA25650.064.8717043
Produced by FastQC (version 0.11.5)