FastQCFastQC Report
Mon 27 Feb 2023
SRR3127368.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127368.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2642954
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263590.9973310167335488No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39500.14945398217297767No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89050.085.3383941
TACGGGT2600.074.110883
AGGGATG46700.073.665765
ACGGGTA3350.071.547894
TAGGGCA14150.071.409094
ATAGGGC15650.071.1713943
CGTAGGG7250.070.695222
TATAGGG23500.070.433032
AGTAGGG46800.069.628812
TAGAGGG51000.069.334482
GAATAGG15450.068.8312761
AGGGCAT24400.068.376995
AGGGTAC11450.067.7251365
ATAGGGA28150.067.448833
AAGAGGG98200.067.229092
CGAAGGG25950.067.2261352
TAGGGAT21100.067.043324
ATAGCGG5350.065.964961
AAGGGCA39200.065.340424
AAGGGAT53250.065.134074
Produced by FastQC (version 0.11.5)