FastQCFastQC Report
Mon 27 Feb 2023
SRR3127367.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127367.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3111035
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT438571.4097237735994612No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36040.11584569122494603No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT150800.086.520581
TAGGGCA17900.071.4292144
TAGAGGG58200.070.429322
ATAGAGG20650.070.127511
ACGGGTA2950.070.111734
ATAGGGC19950.069.273073
AGGGATG47850.068.668145
AGGGTAC14050.068.251765
AGTAAGG19000.067.061521
ATGCGGG17050.066.994932
ATAAGGG46350.066.630892
AGTAGGG50900.066.585172
AGATAGG18000.066.346641
AAGAGGG115400.066.273782
TAAGGGA26200.066.024723
TAGGGTA11200.065.893364
TATAGGG26400.065.880642
CGTAGGG8850.065.862532
TAAGAGG24300.065.592041
AGGGAAT30000.064.712595
Produced by FastQC (version 0.11.5)