FastQCFastQC Report
Mon 27 Feb 2023
SRR3127366.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127366.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2623847
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361561.377976688427336No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30970.1180327968818304No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT113750.087.577971
TAGGGCA13800.071.7356644
TAGGGTA8800.070.710874
CGTAGGG7750.069.950542
ACGGGTA2700.069.837894
AAGGGTA17150.068.718054
AGTAGGG42000.068.578322
TAGAGGG47000.067.90252
ATAGCGG5650.067.622151
ATAGGGA22350.066.861593
TAACGGG7500.066.625362
AGGGTAT16100.066.549455
AGGGATG39000.066.2725455
TAAGAGG20150.066.246621
GACCGAT1350.066.138658
GTAGGGT10200.066.089243
TAGCGGG13650.065.962282
ATAGAGG17500.065.496881
AAGAGGG98650.065.227462
ATAGGGC13900.064.775913
Produced by FastQC (version 0.11.5)