FastQCFastQC Report
Mon 27 Feb 2023
SRR3127365.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127365.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718900
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351951.2944573172974365No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31380.11541432196844313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT115850.086.81951
TAGGGCA13900.072.353644
ACGGGTA2700.071.364384
AGTAGGG40750.071.199112
ATAGGGC15150.070.106333
GGTAAGG15100.068.573221
AAGGGTA18850.068.0632554
TAGACGG5000.067.7752761
ATAGAGG19350.067.6196061
AGGGATG37450.067.5136955
CGTAGGG6350.067.3883362
AGGGCAT24950.067.244865
AAGAGGG99100.066.668352
GAATAGG15750.066.639711
TAGAGGG48950.066.380792
TATAGGG22950.066.181552
AGTAAGG14050.065.9931261
AGGGAAT28300.065.59525
ATAGGGA24400.065.29373
CGAAGGG29150.065.0105062
Produced by FastQC (version 0.11.5)