FastQCFastQC Report
Mon 27 Feb 2023
SRR3127364.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127364.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2945601
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT434901.4764389338542456No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT142950.085.6176451
ATAGCGG5400.074.011371
CGTAGGG8550.073.120782
ATAGGGC17800.072.0937963
GAATAGG18250.071.108171
TAGGGCA17200.070.236034
ACGGGTA2150.069.962744
AGTAGGG51050.068.506612
AGTAAGG21800.067.72461
GTACGGG6550.067.4593052
AGGGATG43950.066.73925
ATAGAGG22350.066.4787451
ATAAGGG43700.066.368042
ATAGGGA26550.066.038863
TAGAGGG56650.065.800412
AATAGGG48350.065.235092
AGGGATA33250.065.031165
TATAGGG25950.064.305222
GGTAAGG17550.064.299471
ATGCGGG14200.064.219762
Produced by FastQC (version 0.11.5)