FastQCFastQC Report
Mon 27 Feb 2023
SRR3127363.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127363.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2528524
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355221.4048512096385084No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT117900.087.281191
AGGGATG38200.071.228695
TAGGGCA13950.070.953684
GTACGGG7200.070.700282
ATAGGGC15250.069.541263
CGTAGGG6450.068.690712
AGTAGGG43900.067.855092
GAATAGG14800.067.5544361
GAGGGAT33100.067.496344
ATAGGGA21450.067.019693
TAGAGGG46150.066.58952
TAGGGCG3050.066.450534
AGGGTAC12800.066.084915
AAGAGGG91550.065.796442
ATAGCGG5750.065.614811
AAGGGTA19350.065.524124
ATAGAGG16900.065.2993551
AGAGGGC37500.064.9814153
CGAAGGG26650.064.554352
AGGGAAT25650.064.4903955
Produced by FastQC (version 0.11.5)