FastQCFastQC Report
Mon 27 Feb 2023
SRR3127362.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127362.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566240
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344551.3426257871438367No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT113100.087.686561
TAGGGCA14600.071.459874
ATAGGGC15900.070.641983
AGTAGGG45200.070.126132
CGTAGGG8000.069.954452
GAATAGG17300.069.92271
ATAGGGA24900.068.701173
AGGGATG38500.068.113885
ATAGCGG5850.067.5856551
AGGGCAT26350.067.06095
GGTAAGG15200.066.267611
ATGCGGG13100.066.054882
TAGGGCG3700.066.0486154
AGAGGGC35550.065.702053
AATAGGG42050.065.537512
TAGAGGG48450.065.5191652
AGTAAGG15850.065.034831
AGGGTAC11800.064.520115
TAAGGGA25300.064.2713853
AAGAGGG92150.064.1502462
Produced by FastQC (version 0.11.5)