FastQCFastQC Report
Mon 27 Feb 2023
SRR3127361.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127361.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2678976
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311441.162533744236604No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC52340.19537315750495712No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC27140.10130736520222651No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT101950.086.610651
AGGGATG61000.077.604985
TATAGGG25750.073.390322
GAATAGG16800.071.646281
ATAGCGG7650.071.295071
CGTAGGG7050.070.6816562
AGATAGG18250.070.333581
TAGCGGG16250.069.1408162
TAACGGG8850.069.054192
TAGAGGG53950.068.924722
TAGGGCA15150.068.8858954
GGATGGC54150.068.4977957
TAAGGGA28600.068.378143
AGTAGGG49100.068.3607252
ATAGGGA28500.068.288163
AAGGGAT58900.068.160464
GAGGGAT40450.068.103444
ATAGAGG21950.067.688591
GGTAAGG16700.067.289051
ATAGGGC15100.067.246053
Produced by FastQC (version 0.11.5)