FastQCFastQC Report
Mon 27 Feb 2023
SRR3127360.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127360.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2312365
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262311.134379736763011No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43780.18932997169564492No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC23460.10145457140200617No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85850.087.255241
AGGGATG49650.075.7191245
ACGGGTA2100.074.078684
TATAGGG19750.073.754752
GGTAAGG13950.073.716321
CTACGAC8950.072.983825
CGTAGGG6950.072.576762
TAGGGCA12150.070.226484
ATAGAGG18550.070.1853561
TAGAGGG44950.069.741392
AGTACGG3950.069.264741
GAGGGAT34200.069.05744
AGGGTAC11450.068.950695
AAGGGAT48550.068.842344
GCATAGG7300.068.4958651
AGGGAAT25500.068.370475
AGAGGGA58650.068.239893
TAACGGG9850.067.959592
GAATAGG14550.067.4344251
GGATGGC44250.067.011747
Produced by FastQC (version 0.11.5)