FastQCFastQC Report
Mon 27 Feb 2023
SRR3127359.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127359.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2347704
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253551.0799913447351115No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45370.19325264172996257No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC24010.10227013286172362No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80850.085.243151
AGGGATG51100.078.177855
CGTAGGG6700.072.300982
GAATAGG14650.071.658171
AAGGGTA14300.070.991014
GGATGGC47150.070.571277
AGGGAAT25350.070.2662665
GGTAAGG13900.070.1058041
TAAGGGA22950.070.037253
TAGGGCG2350.069.998034
ATAGGGA24200.069.332763
TAGGGCA12500.068.806064
GAGGGAT34350.068.5482944
AAGGGAT50350.068.3277744
AGTAGGG39600.068.289572
TAGAGGG46550.068.0960542
AGGGCAT20650.068.051365
GTAGGGA20450.068.027433
CTACGAC7900.067.820885
AGATAGG15000.067.789271
Produced by FastQC (version 0.11.5)