FastQCFastQC Report
Mon 27 Feb 2023
SRR3127357.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127357.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934697
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189680.9804119197993278No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC55390.28629806114342454No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70850.085.6010741
AGGGATG41800.078.809655
GTACGGG4400.077.1376652
CGGGATG9450.076.581985
TAGGGCA13000.073.610434
TAAGGGA20650.071.679723
GGATGGC38250.071.626687
CGTAGGG6450.071.6230852
AGAGGGC31350.071.423763
CTACGAC10950.071.241275
ATGCGGG8350.071.1329352
TAGAGGG34300.070.3659442
TACGACC11100.069.431826
ATAGCGG3600.069.430691
AGGGAAT22100.069.108075
ATAGGGC13350.068.855723
CGCTACG11300.068.8322753
TATAGGG15900.068.4859542
AGTAGGG31950.068.459512
AAGGGAT42200.068.252014
Produced by FastQC (version 0.11.5)